RNA 1 and RNA 2 Genomic Segments of Chronic Bee Paralysis Virus Are Infectious and Induce Chronic Bee Paralysis Disease

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RNA 1 and RNA 2 Genomic Segments of Chronic Bee Paralysis Virus Are Infectious and Induce Chronic Bee Paralysis Disease

Chronic bee paralysis virus (CBPV) causes an infectious and contagious disease of adult honeybees. Its segmented genome is composed of two major positive single-stranded RNAs, RNA 1 (3,674 nt) and RNA 2 (2,305 nt). Three minor RNAs (about 1,000 nt each) have been described earlier but they were not detected by sequencing of CBPV genome. In this study, the results of in vivo inoculation of the t...

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Molecular identification and phylogenetic analysis of chronic bee paralysis virus in Iran

Chronic bee paralysis virus (CBPV) is an unclassified polymorphic single-stranded RNA virus. Among the viruses infecting honeybees, CBPV is known to induce significant losses in honeybee colonies. In this study, a total number of eighty-nine suspected apiaries from four regions of Iran (including Mazandaran, Khorasan Razavi, Hormozgan, and Kurdistan) were sampled and submitted for molecular ide...

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First molecular detection of Chronic Bee Paralysis Virus (CBPV) in Iran

  Among the viruses infecting honey bees, chronic bee paralysis virus (CBPV) is known to induce significant losses in honey bee colonies. CBPV is an unclassified polymorphic single stranded RNA virus. Using RT-PCR, the virus infections in honey bees can be detected in a rapid and accurate manner. Bee samples were collected from 23 provinces of Iran, between July-September 2011 and 2012. A tota...

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Molecular detection of Acute Bee Paralysis Virus in Iran

Acute bee paralysis virus (ABPV) is a small single stranded RNA virus recently classified within the family Dicistroviridae, genus Cripavirus. Here, we describe the first study of ABPV in unhealthy bee colonies, which has been an unusual loss in adult bee population and significant honey bee mortality during the year. The aim of this study was evaluation of ABPV infection in honey bee colonies ...

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Detection of Chronic bee paralysis virus ( CBPV ) genome 1 and its replicative RNA form in various hosts and possible 2 ways of spread . 3 4

22 Detection of Chronic bee paralysis virus (CBPV) is reported for the first time in two 23 species of ants (Camponotus vagus and Formica rufa) and in Varroa destructor. A 24 quantitative real-time PCR (qPCR) method was used to detect and quantify CBPV in 25 infected bees, ants and mites. A minus-strand-specific RT-PCR was used to assess viral 26 replication. These results suggest a new way by ...

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ژورنال

عنوان ژورنال: Journal of Immunology Research

سال: 2015

ISSN: 2314-8861,2314-7156

DOI: 10.1155/2015/423493